Coding
Part:BBa_K317002:Design
Designed by: Yoshiyuki MURAKAMI Group: iGEM10_Tokyo-NoKoGen (2010-10-19)
Tar (StTar)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 1480
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1282
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 997
Illegal SapI.rc site found at 1498
Design Notes
The coding sequence was amplified with primer sets which were designed based on 'Standard part fabrication' described in http://openwetware.org/wiki/Synthetic_Biology:BioBricks/Part_fabrication
Source
Salmonella Typhimurium